Journal: Cell Death & Disease

Article Title: Protein synthesis inhibition and loss of homeostatic functions in astrocytes from an Alzheimer’s disease mouse model: a role for ER-mitochondria interaction

doi: 10.1038/s41419-022-05324-4

Figure Lengend Snippet: a Cells were pulsed with puromycin (4 µM, 1.5 h), fixed and analysed by IF with anti-puromycin antibody (green) and with DAPI to stain nuclei (blue). Images were acquired with Leica Thunder imager 3D live cell microscope, scale bar = 25 µm. Data are expressed as mean ± SEM, WT-iAstro cells n = 40, 3Tg-iAstro cells n = 40, from 4 independent experiments; **** p < 0.05 by unpaired t -test. b WB with anti-puromycin antibody and ponceau staining on cells treated with 4 µM puromycin. Data are expressed as mean ± SEM of 12 independent experiments; **** p < 0.0001 by unpaired t -test.

Article Snippet: Images were acquired using a FV-1000 Olympus laser confocal scanning system, Zeiss 710 confocal laser scanning microscope, Leica SP8 LIGHTNING Confocal Microscope imaging systems and Leica Thunder imager 3D live cell.

Techniques: Staining, Microscopy